Andrographolide, A Novel Repressor
of Hepcidin Gene Expression
Published: May 1, 2018 | DOI: https://doi.org/10.7860/JCDR/2018/34989.11525
Vadoud Malekzadeh, Farideh Manafi, Reza Alipanah-Moghadam, Ali Nemati, Arash Mehri, Firouz Norouzi, Mohammad Mohammadzadeh-Vardin, Firouz Amani
1. Instructor, Department of Anatomical Sciences, Research Laboratory for Embryology and Stem Cells, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
2. Master of Science, Department of Clinical Biochemistry, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
3. Assistant Professor, Department of Clinical Biochemistry, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
4. Associate Professor, Department of Clinical Biochemistry, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
5. Master of Science, Department of Clinical Biochemistry, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
6. Master of Science, Department of Clinical Biochemistry, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
7. Assistant Professor, Department of Anatomical Sciences, Research Laboratory for Embryology and Stem Cells, School of Medicine, Ardabil Univer
Correspondence
Dr. Reza Alipanah-Moghadam,
Daneshgah Street, Ardabil University of Medical Sciences, Ardabil, Iran.
E-mail: alipanahreza9@gmail.com
Introduction: Hepcidin is the most important factor in iron metabolism and plays a potential role in erythropoiesis. It is a small peptide hormone which is cysteine-rich and is mostly secreted by hepatic cells.
Aim: The purpose of this study was to evaluate the effect of andrographolide on the expression of hepatic hepcidin in an iron overload model.
Materials and Methods: For the current study, 48 male Wistar rats were used in six groups. These groups included control group, andrographolide with 3.5 and 7 mg/kg doses groups, iron plus andrographolide groups with 3.5 and 7 mg/kg doses, and iron group. Hepcidin gene expression was performed by real-time method. Iron serum levels were measured by photometry. We used ANOVA and Kruskal Wallis to compare the means of the factors under investigation.
Results: The results revealed that the quantitative expression levels of mRNA hepcidin decreased in all groups except in iron group compared with the control group. This decrease in andrographolide 7 mg/kg, iron plus andrographolide 3.5 mg/kg, and iron plus andrographolide 7 mg/kg groups was significant compared to the control group (p<0.05). The quantitative expression level of mRNA hepcidin significantly increased in iron group as compared to the control group (p<0.05). The findings also indicated that serum concentration of iron in groups with secondary iron overload significantly increased compared with the control group (p<0.05).
Conclusion: Andrographolide with 3.5 and 7 mg/kg doses decreases the expression of hepcidin and increases iron serum levels in secondary iron overload model.
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